Down-regulation of an anionic peroxidase in transgenic aspen and its effect on lignin characteristics.
Identifieur interne : 004465 ( Main/Exploration ); précédent : 004464; suivant : 004466Down-regulation of an anionic peroxidase in transgenic aspen and its effect on lignin characteristics.
Auteurs : Yahong Li [Japon] ; Shinya Kajita ; Shinya Kawai ; Yoshihiro Katayama ; Noriyuki MorohoshiSource :
- Journal of plant research [ 0918-9440 ] ; 2003.
Descripteurs français
- KwdFr :
- Gènes de plante (génétique), Gènes rapporteurs (génétique), Hybridation génétique (MeSH), Lignine (métabolisme), Peroxidases (génétique), Peroxidases (métabolisme), Populus (enzymologie), Populus (génétique), Populus (métabolisme), Régions promotrices (génétique) (génétique), Régulation de l'expression des gènes végétaux (MeSH), Régulation négative (MeSH), Tabac (génétique), Végétaux génétiquement modifiés (MeSH).
- MESH :
- enzymologie : Populus.
- génétique : Gènes de plante, Gènes rapporteurs, Peroxidases, Populus, Régions promotrices (génétique), Tabac.
- métabolisme : Lignine, Peroxidases, Populus.
- Hybridation génétique, Régulation de l'expression des gènes végétaux, Régulation négative, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Down-Regulation (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Plant (genetics), Genes, Reporter (genetics), Hybridization, Genetic (MeSH), Lignin (metabolism), Peroxidases (genetics), Peroxidases (metabolism), Plants, Genetically Modified (MeSH), Populus (enzymology), Populus (genetics), Populus (metabolism), Promoter Regions, Genetic (genetics), Tobacco (genetics).
- MESH :
- chemical , genetics : Peroxidases.
- chemical , metabolism : Lignin, Peroxidases.
- enzymology : Populus.
- genetics : Genes, Plant, Genes, Reporter, Populus, Promoter Regions, Genetic, Tobacco.
- metabolism : Populus.
- Down-Regulation, Gene Expression Regulation, Plant, Hybridization, Genetic, Plants, Genetically Modified.
Abstract
It is generally accepted that peroxidases catalyze the final step in the biosynthesis of lignin. In this study, to examine how expression of prxA3a, a gene for an anionic peroxidase, might be related to lignification in plant tissues, we produced transgenic tobacco plants that harbored a gene for beta-glucuronidase (GUS) fused to the prxA3a promoter. Histochemical staining for GUS activity indicated that the prxA3a promoter was active mainly in the lignifying cells of stem tissues. Further, to examine the effects of suppressing the expression of prxA3a, we transferred an antisense prxA3a gene construct into the original host, hybrid aspen ( Populus sieboldii x P. gradidentata), under the control of the original promoter of the prxA3a gene. Eleven transformed aspens were obtained and characterized, and the stable integration of the antisense construct was confirmed by PCR and Southern blotting analysis in all these lines. Assays of enzymatic activity showed that both total peroxidase activity and acidic peroxidase activity were lower in most transgenic lines than in the control plants. In addition, the reduction of peroxidase activity was associated with lower lignin content and modified lignin composition. Transgenic lines with the highest reduction of peroxidase activity displayed a higher syringyl/vanillin (S/V) ratio and a lower S+V yield, mainly because of a decreased amount of V units. Thus, our results indicate that prxA3a is involved in the lignification of xylem tissue and that the down-regulation of anionic peroxidase alters both lignin content and composition in hybrid aspen.
DOI: 10.1007/s10265-003-0087-5
PubMed: 12836039
Affiliations:
Links toward previous steps (curation, corpus...)
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Shinya" uniqKey="Kajita S" first="Shinya" last="Kajita">Shinya Kajita</name></author><author><name sortKey="Kawai, Shinya" sort="Kawai, Shinya" uniqKey="Kawai S" first="Shinya" last="Kawai">Shinya Kawai</name></author><author><name sortKey="Katayama, Yoshihiro" sort="Katayama, Yoshihiro" uniqKey="Katayama Y" first="Yoshihiro" last="Katayama">Yoshihiro Katayama</name></author><author><name sortKey="Morohoshi, Noriyuki" sort="Morohoshi, Noriyuki" uniqKey="Morohoshi N" first="Noriyuki" last="Morohoshi">Noriyuki Morohoshi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:12836039</idno><idno type="pmid">12836039</idno><idno type="doi">10.1007/s10265-003-0087-5</idno><idno type="wicri:Area/Main/Corpus">004444</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004444</idno><idno type="wicri:Area/Main/Curation">004444</idno><idno type="wicri:explorRef" 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type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kajita, Shinya" sort="Kajita, Shinya" uniqKey="Kajita S" first="Shinya" last="Kajita">Shinya Kajita</name></author><author><name sortKey="Kawai, Shinya" sort="Kawai, Shinya" uniqKey="Kawai S" first="Shinya" last="Kawai">Shinya Kawai</name></author><author><name sortKey="Katayama, Yoshihiro" sort="Katayama, Yoshihiro" uniqKey="Katayama Y" first="Yoshihiro" last="Katayama">Yoshihiro Katayama</name></author><author><name sortKey="Morohoshi, Noriyuki" sort="Morohoshi, Noriyuki" uniqKey="Morohoshi N" first="Noriyuki" last="Morohoshi">Noriyuki Morohoshi</name></author></analytic><series><title level="j">Journal of plant research</title><idno type="ISSN">0918-9440</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Down-Regulation (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Plant (genetics)</term><term>Genes, Reporter (genetics)</term><term>Hybridization, Genetic (MeSH)</term><term>Lignin (metabolism)</term><term>Peroxidases (genetics)</term><term>Peroxidases (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Promoter Regions, Genetic (genetics)</term><term>Tobacco (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Gènes de plante (génétique)</term><term>Gènes rapporteurs (génétique)</term><term>Hybridation génétique (MeSH)</term><term>Lignine (métabolisme)</term><term>Peroxidases (génétique)</term><term>Peroxidases (métabolisme)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Régions promotrices (génétique) (génétique)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Régulation négative (MeSH)</term><term>Tabac (génétique)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peroxidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term><term>Peroxidases</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genes, Plant</term><term>Genes, Reporter</term><term>Populus</term><term>Promoter Regions, Genetic</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Gènes de plante</term><term>Gènes rapporteurs</term><term>Peroxidases</term><term>Populus</term><term>Régions promotrices (génétique)</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lignine</term><term>Peroxidases</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Down-Regulation</term><term>Gene Expression Regulation, Plant</term><term>Hybridization, Genetic</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Hybridation génétique</term><term>Régulation de l'expression des gènes végétaux</term><term>Régulation négative</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">It is generally accepted that peroxidases catalyze the final step in the biosynthesis of lignin. In this study, to examine how expression of prxA3a, a gene for an anionic peroxidase, might be related to lignification in plant tissues, we produced transgenic tobacco plants that harbored a gene for beta-glucuronidase (GUS) fused to the prxA3a promoter. Histochemical staining for GUS activity indicated that the prxA3a promoter was active mainly in the lignifying cells of stem tissues. Further, to examine the effects of suppressing the expression of prxA3a, we transferred an antisense prxA3a gene construct into the original host, hybrid aspen ( Populus sieboldii x P. gradidentata), under the control of the original promoter of the prxA3a gene. Eleven transformed aspens were obtained and characterized, and the stable integration of the antisense construct was confirmed by PCR and Southern blotting analysis in all these lines. Assays of enzymatic activity showed that both total peroxidase activity and acidic peroxidase activity were lower in most transgenic lines than in the control plants. In addition, the reduction of peroxidase activity was associated with lower lignin content and modified lignin composition. Transgenic lines with the highest reduction of peroxidase activity displayed a higher syringyl/vanillin (S/V) ratio and a lower S+V yield, mainly because of a decreased amount of V units. Thus, our results indicate that prxA3a is involved in the lignification of xylem tissue and that the down-regulation of anionic peroxidase alters both lignin content and composition in hybrid aspen.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12836039</PMID><DateCompleted><Year>2003</Year><Month>09</Month><Day>12</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0918-9440</ISSN><JournalIssue CitedMedium="Print"><Volume>116</Volume><Issue>3</Issue><PubDate><Year>2003</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Journal of plant research</Title><ISOAbbreviation>J Plant Res</ISOAbbreviation></Journal><ArticleTitle>Down-regulation of an anionic peroxidase in transgenic aspen and its effect on lignin characteristics.</ArticleTitle><Pagination><MedlinePgn>175-82</MedlinePgn></Pagination><Abstract><AbstractText>It is generally accepted that peroxidases catalyze the final step in the biosynthesis of lignin. In this study, to examine how expression of prxA3a, a gene for an anionic peroxidase, might be related to lignification in plant tissues, we produced transgenic tobacco plants that harbored a gene for beta-glucuronidase (GUS) fused to the prxA3a promoter. Histochemical staining for GUS activity indicated that the prxA3a promoter was active mainly in the lignifying cells of stem tissues. Further, to examine the effects of suppressing the expression of prxA3a, we transferred an antisense prxA3a gene construct into the original host, hybrid aspen ( Populus sieboldii x P. gradidentata), under the control of the original promoter of the prxA3a gene. Eleven transformed aspens were obtained and characterized, and the stable integration of the antisense construct was confirmed by PCR and Southern blotting analysis in all these lines. Assays of enzymatic activity showed that both total peroxidase activity and acidic peroxidase activity were lower in most transgenic lines than in the control plants. In addition, the reduction of peroxidase activity was associated with lower lignin content and modified lignin composition. Transgenic lines with the highest reduction of peroxidase activity displayed a higher syringyl/vanillin (S/V) ratio and a lower S+V yield, mainly because of a decreased amount of V units. Thus, our results indicate that prxA3a is involved in the lignification of xylem tissue and that the down-regulation of anionic peroxidase alters both lignin content and composition in hybrid aspen.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yahong</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Environmental Symbiotic Production Systems, Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kajita</LastName><ForeName>Shinya</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Kawai</LastName><ForeName>Shinya</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Katayama</LastName><ForeName>Yoshihiro</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Morohoshi</LastName><ForeName>Noriyuki</ForeName><Initials>N</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2003</Year><Month>03</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>J Plant Res</MedlineTA><NlmUniqueID>9887853</NlmUniqueID><ISSNLinking>0918-9440</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.7</RegistryNumber><NameOfSubstance UI="C120494">anionic peroxidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015536" MajorTopicYN="Y">Down-Regulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="N">Genes, Reporter</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006824" MajorTopicYN="N">Hybridization, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2002</Year><Month>11</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2003</Year><Month>01</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>7</Month><Day>2</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>9</Month><Day>13</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>7</Month><Day>2</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12836039</ArticleId><ArticleId IdType="doi">10.1007/s10265-003-0087-5</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Plant Physiol. 1997 Jul;114(3):957-68</Citation><ArticleIdList><ArticleId 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name="Japon"><region name="Région de Kantō"><name sortKey="Li, Yahong" sort="Li, Yahong" uniqKey="Li Y" first="Yahong" last="Li">Yahong Li</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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